Osteoclasts express high levels of p60c‐src, preferentially on ruffled border membranes
- 16 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 313 (1) , 85-89
- https://doi.org/10.1016/0014-5793(92)81190-w
Abstract
Expression of p60c-src , the normal cellular counterpart of the transforming protein of Rous sarcoma virus (RSV), p60c-src , was examined in mouse and rat authentic osteoclasts and mouse osteoclast-like multinucleated cells (MNCs) formed in vitro. In co-cultures of mouse osteoblastic cells and spleen cells, the expression of p60c-src strikingly increased on day 5 in parallel with the appearance of MNCs in the presence of 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). Immunohistochemical examination confirmed the high level expression of p60c-src in both mouse authentic osteoclasts and MNCs. Electron microscopic examination revealed that p60c-src was primarily localized on ruffled border membranes and vacuoles, but not on the clear zone in rat authentic osteoclasts. These results suggest that p60 c-src is important in osteoclastic bone resorption.Keywords
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