FURTHER STUDIES OF ANTIGEN STIMULATION OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN RABBIT SPLEEN CELL CULTURES .I. EFFECTS OF CENTRIFUGED PROTEINS

  • 1 January 1968
    • journal article
    • research article
    • Vol. 14  (3) , 409-+
Abstract
The rate of DNA synthesis, as measured by the uptake of [14C]-thymidine by spleen cell suspensions from rabbits previously immunized to heterologous proteins, was specifically stimu- lated by the deposits obtained after high-speed centrifugation of solutions of these antigens. The deposited material was much more stimulatory than the original protein or the supernatants from centrifuged preparations. Recombination of the centrifuged deposit with the supernatant resulted in restoration of the lost stimulatory activity.