DNA gel electrophoresis: Effect of field intensity and agarose concentration on band inversion

Publisher Website
Google Scholar
Abstract
We study the effect of electric field intensity and agarose gel concentration on the anomalous electrophoretic mobility recently predicted by the biased reptation model and experimentally observed for linear DNA fragments electrophoresed in continuous electric fields. We show that high fields and low agarose concentrations eliminate the physical mechanism responsible for anomalous DNA mobility and band inversion, in good agreement with theory, thus restoring the monotonic mobility–size relationship necessary for unambiguous interpretation of the results of DNA gel electrophoresis.