STUDIES ON THE FORMAION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE

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Abstract
The biosynthesis of biotin-s35 from H2S35O4 and desthiobiotin by a washed cell suspension of biotin-and S-deficient S. cerevisiae was investigated by 2 methods. The biotin and S-deficient cell suspension was incubated with sulfate-S35 and D-glucose; L-methionine-S35 was found to be produced predominantly. Experiments with L-methionine-S35 and desthiobiotin showed the formation of biotin-S35 in a good yield. Using 140 [mu]c of L-methionine-S35, 0.26 [mu]c of biotin-S35 was obtained by the biotin-avidin complex method, followed by autoradiography, bioautography, and paper chromatoscanning. From these findings, it is concluded that 70% of the radioactivity in the biotin concentrate was located in biotin-related compounds, i.e., biotin-D-sulfoxide, biotin-L-sulfoxide, biocytin, and biocytin sulfoxide.

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