Biosynthesis of sucrose phosphate with sugar cane leaf chloroplasts.

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Abstract
The reactions leading to the formation of sucrose and sucrose-P were investigated with chloroplasts from sugarcane leaves as a source of enzyme. Chloroplasts isolated from these leaves synthesized sucrose from UDP-D-glucose and D-fructose; sucrose was also formed when D-fructose 6-P was substituted for D-fructose as the glucose acceptor; but sucrose 6-P was not formed. When acetone-extracted chloroplasts were used, sucrose-P and sucrose were formed from UDP-D-glucose and D-fructose 6-P; only sucrose was synthesized when D-fructose was used as an acceptor. Sugarcane chloroplasts contain phosphatase capable of hydrolyzing D-fructose 6-P as well as sucrose-P.