Biosynthesis of Sucrose and Sucrose-Phosphate by Sugar Beet Leaf Extracts

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Abstract
Cell-free preparations from sugar beet leaf tissue are shown to contain active systems for the synthesis of sucrose and sucrose-phosphate through the interaction of uridine diphosphoglucose and fructose, and uridine diphosphoglucose and fructose-6-phosphate respectively. Methods for preparing and assaying the enzyme mixture, for demonstrating maximum activity in the presence of invertase, and the differentiating sucrose and sucrose-phosphate are described.