The TolC protein of Escherichia coli K12 is synthesised in a precursor form
- 13 June 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 156 (2) , 307-310
- https://doi.org/10.1016/0014-5793(83)80518-3
Abstract
We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse—chase experiment, followed by immunoprecipitation with anti‐TolC antibody and SDS—PAGE of the immunoprecipitate. This showed that TolC protein was originally synthesised in a precusor form (M r 54 500) which could be chased into the mature form (M r 52 000). DNA sequencing of a portion of the cloned tolC gene showed that the N‐terminus of the mature rotein was preceded by a typical signal sequence of 22 residues (M r 2542). The initiator Met was preceded by a Shine—Dalgarno sequence, with the correct spacing.Keywords
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