REGENERATION OF BLOOD-FORMING ORGANS AFTER AUTOLOGOUS LEUKOCYTE TRANSFUSION IN LETHALLY IRRADIATED DOGS .2. DISTRIBUTION AND CELLULARITY OF MARROW IN IRRADIATED AND TRANSFUSED ANIMALS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 47  (4) , 593-601
Abstract
Dogs were given transfusions of cryopreserved autologous mononuclear blood leukocytes after 1200 R (midline dose) whole-body X-irradiation. Bone marrow repopulation was studied by means of histomorphological methods at 9 and 10 days after transfusion of an average of 3 .times. 109, 7 .times. 109, 13 .times. 109 and 31 .times. 109 cells. The return of marrow cellularity to normal values was related to the number of cells transfused. With low cell doses (3 .times. 109 and 7 .times. 109), the marrow regeneration at 10 days was focal. There were groups of cells (colonies) showing either erythropoiesis, myelopoiesis or megakaryocytopoiesis in the osteal niches of the trabecular bones. Frequently such niches were seen showing complete cellular recovery next to niches with complete aplasia. With higher cell doses, all niches showed hemopoietic regeneration, and the cellularity approached normal values. No hemopoietic regeneration was observed in those skeletal parts that do not show hemopoiesis, even under normal circumstances.