Phosphoinositides. 5. The inositol lipids of ox brain

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Abstract
The "diphosphoinositide" fraction was prepared from ox brain by the method of Folch (1949) and hydrolysed under mild alkaline conditions. The hydrolysis products were separated by ion-exchange chro-matography and analysed. The major hydrolysis product was glyceryl-phosphorylinositol diphosphate. Glycerylphosphorylinositol monophos-phate and glycerylphosphorylinositol were also present. It is concluded that ox brain containes 3 phosphoinositides phosphatidylinositol, diphosphoinositide [l-phosphatidyl(inositol 4-phosphate)] and triphospho-inositide [1-phosphatidyl(inositol 4,5-diphosphate)]. Inositol di-and tri-phosphate were also found in hydrolysates of the Folch "diphosphoinositide" fraction. The free diphosphates occur in ox brain. The triphosphate may come from a new type of phosphoinositide.