Underestimation of metabolic rates owing to reincorporation of 14CO2 in the perfused rat liver
- 15 October 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 208 (1) , 231-234
- https://doi.org/10.1042/bj2080231
Abstract
14CO2 production by perfused rat livers was simulated by infusing NaH14CO3 into the perfusate. Recovery of label as 14CO2 gas + perfusate bicarbonate was 45–85%. Rates of 14CO2 exchange in the liver are 3–70 times greater than net rates of CO2 production. Therefore 14CO2 reincorporation can lead to significant underestimations of rates of oxidation of 14C-labelled substrates in liver.This publication has 12 references indexed in Scilit:
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