Preparation of radiolabeled tetracosa mono‐ and dienoic acid methyl esters from rat erythrocyte lipids by thin layer chromatography

Publisher Website
Google Scholar
Abstract
An easy method of obtaining pure fatty acid methyl esters (FAME) of tetracosa mono- and dienoic acids (24∶1, 24∶2) using thin layer chromatography (TLC) is described. The total lipids isolated from rat erythrocytes were treated with methanolic-NaOH. Sphingomyelin was unaffected by this treatment and was separated from FAME of glycerolipids and cholesterol by TLC. FAME of sphingomyelin were then prepared by acid methanolysis. These esters migrated into 2 distinct bands on TLC. The slow moving band contained FAME of 16∶0, 16∶1, 18∶0, 18∶1, 19∶0 and 20∶0 whereas the fast moving band contained FAME of 22∶0, 23∶0, 24∶0, 24∶1 and 24∶2. After AgNO3-TLC, the FAME of the fast moving band separated into 3 species; esters of saturated acids, 24∶1 and 24∶2, respectively. With erythrocyte lipids of rats fed a fat-free diet and injected with14C-18∶1, this method yielded14C-24∶1. From rats injected with14C-18∶2 and maintained on a corn oil diet,14C-24∶2 was obtained.