Feulgen stain stability in relation to three mounting media and exposure to light

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Abstract
Fading of Feulgen-stained rat liver nuclei was studied with sections mounted in Eukitt, Clearmount, and Permount, each under conditions of light and dark storage. Repeated microdensitometric measurements were made on selected nuclei over a 160 day post-staining period. In all three media the stain faded significantly when exposed to daily laboratory lighting, but faded least in Eukitt. Slides stored in darkness faded less than those exposed to light but, again, Eukitt-mounted preparations showed the least fading. Eukitt's low refractive index, however, imparts a high refractility to the cytoplasm which may be undesirable for scanning microdensitometry, and its apparently long stabilizing period may be disadvantageous if care is not taken to measure slides at comparable times during the period following mounting.