T cell responses to alloantigens. I. Studies of in vivo and in vitro immunologic memory and suppression by limit dilution analysis.
Open Access
- 1 June 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 124 (6) , 2936-2943
- https://doi.org/10.4049/jimmunol.124.6.2936
Abstract
A limit dilution technique was used to study the frequency of cytotoxic T cell precursors (f pre Tc) in the peripheral blood lymphocytes (pbl) of naive and alloimunized mice. It was found that alloimmune mice showed a 2- to 5-fold specific increase in the f pre Tc reactive to the immunizing alloantigens. This technique was further adapted for use as a sensitive in vitro assay for alloantigen-specific suppressor T cells. It was found that nonimmunosuppressed B6AF1 mice bearing surviving B10.BR cardiac allografts had circulating alloantigen-specific suppressor cells. In vitro it was shown that the culture of heat-inactivated B10.D2 spleen cells with B6AF1 spleen cells gave rise to alloantigen-specific B6AF1 suppressor cells.This publication has 11 references indexed in Scilit:
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