Determination of the Intraruminal Distribution of Soluble Nitrogen

Abstract
An analytical fractionation procedure was developed which facilitates relatively detailed nitrogen balance studies of the rumen fermentation. The procedure was evaluated in an in vivo experiment using 3 animals and 3 treatments. Regardless of dietary treatment most of the nitrogen (65-78%) was observed in feed residues and microbial cells. Only very small amounts of N (less than 2%) were encountered in the form of dissolved protein. Acceptable recoveries were obtained.