The Deoxyglucose Method in the Ferret Brain. I. Methodological Considerations
Open Access
- 1 February 1989
- journal article
- research article
- Published by SAGE Publications in Journal of Cerebral Blood Flow & Metabolism
- Vol. 9 (1) , 35-42
- https://doi.org/10.1038/jcbfm.1989.5
Abstract
In the brain of the anesthetized ferret, the 2-deoxyglucose (2-DG) transfer rate constants required to determine cerebral glucose utilization by the deoxyglucose method were calculated from regional gray matter time-radioactivity curves measured for 180 min after tracer injection. Results suggest that loss of metabolized tracer from brain occurs at a rate of about 1%/min for the first 180 min after injection if the rate constant of the rate-limiting step for loss of metabolized tracer ( k*4) represents a first-order kinetic process. A simulation experiment shows that, whether k*4 is assumed to be 0 or 0.01 min−1, has a negligible influence on glucose utilization rates obtained in conventional 45 min autoradiographic experiments provided that the entire analysis, including lumped constant determination, is carried out in a consistent way. The 2-DG lumped constant for k*4 = 0 is 0.54, and 0.68 for k*4 = 0.01 min−1.Keywords
This publication has 28 references indexed in Scilit:
- The Deoxyglucose Method in the Ferret Brain. II. Glucose Utilization Images and Normal ValuesJournal of Cerebral Blood Flow & Metabolism, 1989
- Deoxyglucose‐6‐Phosphate Stability In Vivo and the Deoxyglucose Method: Response to Comments of Hawkins and MillerJournal of Neurochemistry, 1987
- Simultaneous in vivo measurement of lumped constant and rate constants in experimental cerebral ischemia using F-18 FDG.Stroke, 1987
- Measurement of Brain Deoxyglucose Metabolism by NMRScience, 1986
- Invalidity of Criticisms of the Deoxyglucose Method Based on Alleged Glucose‐6‐Phosphatase Activity in BrainJournal of Neurochemistry, 1986
- Reexamination of Glucose-6-Phosphatase Activity in the Brain in Vivo: No Evidence for a Futile CycleScience, 1985
- An Improved Approach for Measurement of Regional Cerebral Rate Constants in the Deoxyglucose Method with Positron Emission TomographyJournal of Cerebral Blood Flow & Metabolism, 1984
- Alternative Approach to Single-Scan Estimation of Cerebral Glucose Metabolic Rate Using Glucose Analogs, with Particular Application to IschemiaJournal of Cerebral Blood Flow & Metabolism, 1984
- Calculation of cerebral glucose phosphorylation from brain uptake of glucose analogs in vivo: A re-examinationBrain Research Reviews, 1982
- Demonstration of aromatic l-amino acid decarboxylase activity in human brain with l-dopa and l-5-hydroxytryptophan as substrates by high-performance liquid chromatography with electrochemical detectionNeurochemistry International, 1981