Physical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall
- 1 April 1960
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 75 (1) , 12-17
- https://doi.org/10.1042/bj0750012
Abstract
The isolated yeast-cell wall has been separated into 3 fractions: fraction A, soluble in ethylenediamine and water; fraction B, soluble in ethylenediamine and insoluble in water; fraction C, insoluble in both ethylenediamine and water. The physical and chemical characteristics of these fractions have been determined, from which it is concluded that fraction A is a mannan-protein complex and fraction B possibly may be a glucanmannan-protein complex. All 3 fractions contain glucosamine, and it is suggested that this amino sugar might provide the link between the protein and carbohydrate. It is estimated that no more than approximately 9% of the total glucosamine of the cell wall could be present as chitin. The molecular weight of yeast mannan was found to be 5-9 x 104. It contained 1-4% of glucosamine and 1.5% of protein. Yeast gluecan was found to contain 6-0% of glucosamine and 0-8% of protein.Keywords
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