Metabolic roles of peptone and yeast extract for the culture of a recombinant strain ofEscherichia coli
- 1 October 1991
- journal article
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 8 (3) , 165-169
- https://doi.org/10.1007/bf01575849
Abstract
The influence of complex compounds on the growth of a recombinant strain ofEscherichia coli containing the gene encoding glyceraldehyde 3-phosphate dehydrogenase, as well as the production of this enzyme have been studied. Batchwise cultures led to an accumulation of acetate, which was not utilized in a yeast extract-free medium. After glucose exhaustion, growth stopped and enzyme activity decreased. Whereas yeast extract allowed acetate assimilation and growth, peptone stabilized the enzymatic activity. The addition of both compounds resulted in optimal performances for enzyme production.Keywords
This publication has 12 references indexed in Scilit:
- The effect of yeast extract supplementation on the production of lactic acid from whey permeate byLactobacillus helueticusApplied Microbiology and Biotechnology, 1990
- Production of glyceraldehyde-3-phosphate dehydrogenase using genetically engineeredEscherichia coliBiotechnology Letters, 1989
- Utilization of acetate in Escherichia coli: structural organization and differential expression of the ace operonBiochimie, 1989
- The fermentation pathways of Escherichia coliFEMS Microbiology Reviews, 1989
- Use of site-directed mutagenesis to probe the role of Cys149 in the formation of charge-transfer transition in glyceraldehyde-3-phosphate dehydrogenaseProtein Engineering, Design and Selection, 1988
- The effect of organic nitrogen and glucose on the production of recombinant human insulin-like growth factor in high cell densityEscherichia coli fermentationsJournal of Industrial Microbiology & Biotechnology, 1987
- Mass culture of Escherichia coli: Medium development for low and high density cultivation of Escherichia coli B/r in minimal and complex mediaJournal of Biotechnology, 1985
- Acetate formation in continuous culture of Escherichia coli K12 D1 on defined and complex mediaJournal of Biotechnology, 1984
- Molecular cloning of the glyceraldehyde-3-phosphate dehydrogenase genes of Bacillus stearothermophilus and Escherichia coli, and their expression in Escherichia coliGene, 1983
- Regulation of Isocitrate Dehydrogenase Activity in Escherichia coli on Adaptation to AcetateJournal of General Microbiology, 1971