Characterization of non‐adrenergic, non‐cholinergic inhibitory responses of the isolated guinea‐pig trachea: differences between pre‐ and post‐ganglionic nerve stimulation

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Abstract
Differences in the mechanism of non‐adrenergic, non‐cholinergic (NANC) inhibitory responses to preganglionic‐ and post‐ganglionic nerve stimulation were investigated in the guinea‐pig isolated trachea. Stimulation of the vagus nerve at frequencies above 4 Hz elicited NANC relaxation of the trachealis muscle. Responses to low frequencies of stimulation (4–8 Hz) were abolished by the nitric oxide (NO) synthase inhibitor L‐NOARG (10 μM), while a L‐NOARG resistant component was observed at higher stimulus frequencies. The L‐NOARG‐resistant component of NANC inhibitory responses to higher frequencies of vagus nerve stimulation were significantly attenuated by the proteinase α‐chymotrypsin (2 U/ml), suggesting that a neuropeptide such as VIP may contribute to NANC responses. When postganglionic nerves were stimulated by electrical field stimulation (EFS), responses were readily elicited at frequencies below 4 Hz. Like responses to vagus nerve stimulation, responses to low frequency (British Journal of Pharmacology (1999) 128, 458–464; doi:10.1038/sj.bjp.0702786

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