Botulinum neurotoxin light chains inhibit both Ca2+‐induced and GTP analogue‐induced catecholamine release from permeabilised adrenal chromaffin cells
- 20 May 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 386 (2-3) , 137-140
- https://doi.org/10.1016/0014-5793(96)00432-2
Abstract
Using digitonin-permeabilised bovine adrenal chromaffin cells, the effects of botulinum neurotoxin light chains on exocytosis triggered by Ca2+ or by GppNHp were examined. Botulinum neurotoxin D light chain, prepared as a His6-tagged recombinant protein, cleaved VAMP and substantially inhibited catecholamine release due to Ca2+ and GppNHp. Botulinum neurotoxin C1 and E light chains produced partial inhibition of both Ca2+- and GppNHp-induced catecholamine release. These results suggest that Ca 2+-dependent exocytosis and Ca2+-independent exocytosis triggered by a non-hydrolysable GTP analogue occurs via a SNARE-dependent mechanism in chromaffin cells.Keywords
This publication has 49 references indexed in Scilit:
- Blockade by Botulinum Neurotoxin B of Catecholamine Release from Adrenochromaffin Cells Correlates with Its Cleavage of Synaptobrevin and a Homolog Present on the GranulesBiochemistry, 1995
- SNAP-25 is expressed in islets of Langerhans and is involved in insulin release.The Journal of cell biology, 1995
- Mechanisms of intracellular protein transportNature, 1994
- SNAP‐25 is present in a SNARE complex in adrenal chromaffin cellsFEBS Letters, 1994
- Activation of exocytosis by GTP analogues in adrenal chromaffin cells revealed by patch-clamp capacitance measurementFEBS Letters, 1994
- Functional characterization of the catalytic site of the tetanus toxin light chain using permeabilized adrenal chromaffin cellsFEBS Letters, 1993
- Botulinum neurotoxins serotypes A and E cleave SNAP‐25 at distinct COOH‐terminal peptide bondsFEBS Letters, 1993
- SNAP receptors implicated in vesicle targeting and fusionNature, 1993
- Control of exocytosis in adrenal chromaffin cellsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1991
- Effects of Tetanus Toxin on Catecholamine Release from Intact and Digitonin‐Permeabilized Chromaffin CellsJournal of Neurochemistry, 1988