High precursor frequency of human T cells reactive to HLA-DQ molecules expressed on mouse L cell transfectants
- 1 October 1991
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 21 (10) , 2341-2347
- https://doi.org/10.1002/eji.1830211008
Abstract
In humans, the HLA‐DR molecule is a major stimulatory molecule of allogeneic mixed lymphocyte reactions (MLR) and a major restriction molecule for the presentation of soluble antigens to the T cell. Little is known of the biological function of HLA‐DQ. To examine the size of the repertoire of precursor T cells recognizing the autologous or allogeneic HLA‐DQ molecule, the frequency of T cells reactive to HLA‐DQ was estimated in comparison with T cells reactive to HLA‐DR. We made use of a limiting dilution analysis and mouse L cells transfectants expressing the HLA‐DR or ‐DQ molecule, as stimulators. Human T cells recovered from a primary MLR stimulated with allogeneic peripheral blood lymphocytes (PBL) proliferated in response to L cell transfectants expressing HLA class II genes shared by the stimulator cells in the primary MLR. This observation suggested that the HLA class II molecules on L cell transfectants shared to some extent epitopes for alloreactive T cells with those expressed on human PBL. The precursor frequencies of CD4+ T cells reactive to allogeneic or autologous DQ molecules were as high as those of T cells reactive to allogeneic DR molecules and were estimated to be 1/800 ∼ 1/1800. The frequency of the T cells reactive to autologous DR molecules was low (1/7200 ∼ 1/16000). The biological significance of the high frequency of HLA‐DQ‐reactive precursor T cells is discussed.Keywords
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