Morphologic and Functional Heterogeneity Among Rat Alveolar Macrophage Fractions Isolated by Centrifugation on Density Gradients
- 1 September 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Leukocyte Biology
- Vol. 42 (3) , 188-196
- https://doi.org/10.1002/jlb.42.3.188
Abstract
Lavaged rat bronchoalveolar cells were separated into four different density fractions (I—IV) by centrifugation through a discontinuous Percoll gradient. Maximum cell size was found in the lowest density fraction (I) and minimal cell size in the highest density fraction (IV), showing an inverse correlation with cell density. These fractions contained alveolar macrophages (AM) in the proportion of 97% or more by morphologic criteria, while phagocytic AM was approximately 80% in each fraction. Although the proportion of la antigen-positive AM was low in each fraction, it was elevated after incubation with supernatants from concanavalin A (Con A) stimulated spleen cell cultures, with greater la expression in higher density fractions. Differences in these fractions were also noted in several functions, including Fc receptor activity, chemotactic migration, tumoricidal activity, and interleukin 1 (IL-1) production, all of which were greater in higher density fractions (III and IV). Con A-induced T cell proliferation was, however, suppressed by these higher density fractions, whereas only an intermediate density fraction (II) enhanced T cell responses. These results indicate morphologic and functional heterogeneity among rat AM.Keywords
This publication has 7 references indexed in Scilit:
- HETEROGENEITY OF IMMUNOLOGICAL FUNCTION AMONG SUBFRACTIONS OF NORMAL RAT ALVEOLAR MACROPHAGES .2. ACTIVATION AS A DETERMINANT OF FUNCTIONAL-ACTIVITYPublished by Elsevier ,1985
- Heterogeneity of Immunologic Function Among Subfractions of Normal Rat Alveolar Macrophages1–3American Review of Respiratory Disease, 1984
- ENHANCED RELEASE OF A CHEMOATTRACTANT FOR ALVEOLAR MACROPHAGES AFTER ASBESTOS INHALATIONPublished by Elsevier ,1983
- Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages.The Journal of Experimental Medicine, 1980
- Alveolar macrophagesCellular Immunology, 1980
- ALVEOLAR MACROPHAGES .2. INHIBITION OF LYMPHOCYTE-PROLIFERATION BY PURIFIED MACROPHAGES FROM RAT LUNG1979
- Destruction of Tumor Cells by BCG-Activated Alveolar MacrophagesThe Journal of Immunology, 1977