Identification of a novel adhesion molecule in human leukocytes by monoclonal antibody LB‐2

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Abstract
Monoclonal antibody LB‐2 to a surface antigen on human B cells, lymphoblast, monocytes and vascular endothelial cells largely inhibited adhesion among Epstein Barr virus‐immortalized normal B cells (EBV‐B) and concanavalin A‐stimulated blood mononuclear cells (Con A‐BMC) before and after phorbol ester treatment. The antibody inhibited to a lesser extent phorbol ester‐induced aggregation of monocytes, U937 cells and fresh BMC and had virtually no inhibitory effect on the adhesion among enriched T cells and granulocytes. A surface glycoprotein band of 84 kDa was obtained from EBV‐B cells by immunoprecipitation and gel electrophoresis. Immunological and biochemical studies clearly distinguished this molecule from gp90 and associated glycoproteins which also mediate leukocyte adhesion.