A recombinagenic effect of strychnine inSalmonella typhimurium

1 January 1987

journal article
research article
Published by Wiley in Environmental and Molecular Mutagenesis

Vol. 10 (1) , 27-33
https://doi.org/10.1002/em.2850100105

Abstract

The aroC321 allele in Salmonella typhimurium permits a positive selection for genetic duplications. Bacteria that contain a large genetic duplication are detected as tryptophan prototrophs in aroC321 strains and occur at a spontaneous frequency greater than 1/10⁴ cells plated on the selection medium. Duplications originate by a recombinational mechanism, and the induction of duplications by chemicals or radiation may therefore be considered to be a recombinagenic effect. We have found that strychnine is a potent recombinagen in this system; it causes a dose‐dependent increase in the frequency of genetic duplications, and very high frequencies of duplications are recovered at high doses. In contrast, brucine, the 2,3‐dimethoxy derivative of strychnine, caused no increase in duplication frequencies under the identical conditions.

Keywords

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