Synergism of recombinant human interferon gamma with liposome‐encapsulated muramyl tripeptide in activation of the tumoricidal properties of human monocytes

Publisher Website
Google Scholar
Abstract
Freshly isolated human peripheral blood monocytes from healthy volunteers are not cytotoxic to allogeneic A375 melanoma cells, but they were rendered tumoricidal by incubation in vitro with either liposomes containing 5 μg/μtmol phospholipid of muramyl tripeptide phosphatidylethanolamine (liposome-MTP-PE; optimal dose, 500 nmol/ml) or recombinant human interferon gamma (rIFN-γ; optimal dose, 100 U/ml). A combination of sub-threshold concentrations of liposome-MTP-PE (50 nmol/ml) and rIFN-γ (1 or 10 U/ml) also induced significant tumor-cell killing, indicating that the effects of rIFN-γ and liposome-MTP-PE in monocyte activation are synergistic. In contrast to rIFN-γ, recombinant INF-α and IFN-β had additive effects with liposome-MTP-PE in human monocyte activation. Since recombinant human IFN-γ has a synergistic effect with liposome-MTP-PE in monocyte activation, unlike IFN-α or IFN-β, and liposome-MTP-PE as well as rIFN-γ is available at standardized concentrations, this combination could be of clinical value in the treatment of disseminated malignant disease.

This publication has 32 references indexed in Scilit: