PLACENTAL PROTEIN 5 (PP5): DEVELOPMENT OF A RADIOIMMUNOASSAY AND MEASUREMENT OF CIRCULATING LEVELS IN NORMAL PREGNANCY
- 30 April 1981
- journal article
- research article
- Published by Wiley in BJOG: An International Journal of Obstetrics and Gynaecology
- Vol. 88 (5) , 484-491
- https://doi.org/10.1111/j.1471-0528.1981.tb01021.x
Abstract
A specific and sensitive, double antibody, solid phase radioimmunoassay was developed for measurement of placental protein 5 (PP5) in serum. A sensitivity limit of 0.8 μg/1 was achieved by pre-incubation with antiserum and use of a small assay volume. Single serum samples from 244 retrospectively proven normal pregnancies were used to construct a normal range of PP5 concentrations. PP5 was detectable at week 7 and rose to reach a maximum mean value of 54 μg/1 at weeks 36–37. Diurnal variation of PP5 concentration was similar to that encountered from day to day, and slightly greater than variation due to the radioimmunoassay. The decline of serum PP5 concentration post partum was initially rapid (half-life 5–14 minutes), but slowed after 0.5–1 hours; immunoreactive PP5 was still detectable seven days after delivery.This publication has 10 references indexed in Scilit:
- CIRCULATING LEVELS OF PLACENTAL PROTEIN 5 IN THE MOTHER: RELATION TO BIRTH WEIGHTBJOG: An International Journal of Obstetrics and Gynaecology, 1980
- THE VARIATION OF MATERNAL BLOOD LEVELS OF PLACENTAL PROTEIN 5 IN LATE PREGNANCYBJOG: An International Journal of Obstetrics and Gynaecology, 1979
- The radioimmunoassay of placental protein 5 and circulating levels in maternal blood in the third trimester of normal pregnancyClinica Chimica Acta; International Journal of Clinical Chemistry, 1979
- Circulating levels and tissue localization of placental protein five (PP5) in pregnancy and tropho‐blastic disease: Absence of PP5 expression in the malignant trophoblastInternational Journal of Cancer, 1979
- Isolation and Characterization of Placental Proteins with Special Reference to Pregnancy-Specific β1 Glycoprotein and Other Proteins Specific to the PlacentaPublished by Springer Nature ,1979
- Radioimmunoassays of ovine LH and ovine prolactin using polymerized second antiseraAnalytical Biochemistry, 1977
- Isolierung und Charakterisierung des Plazenta-Proteins PP5Archiv für Gynäkologie, 1977
- The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassayBiochemical Journal, 1973
- Nachweis und Charakterisierung von löslichen Antigenen in der menschlichen PlacentaArchiv für Gynäkologie, 1972
- THE ASSESSMENT OF FETAL GROWTHBJOG: An International Journal of Obstetrics and Gynaecology, 1968