Effect of centrifuging shell vials at 3,500 x g on detection of viruses in clinical specimens
- 1 June 1994
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 32 (6) , 1580-2
- https://doi.org/10.1128/jcm.32.6.1580-1582.1994
Abstract
An increase in shell vial centrifugation force to 3,500 x g and a concomitant reduction in spin time to 15 min did not decrease the sensitivity of detecting viruses in clinical specimens compared with the accepted practice of using 700 x g for 40 min. No damage to the cell monolayer (ML) at the higher g force was observed. Toxicity to the ML is decreased with the shorter spin, probably because of reduced time of contact between the specimen and the ML.Keywords
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