Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination
Open Access
- 1 January 2008
- journal article
- Published by Springer Nature in Microbial Cell Factories
- Vol. 7 (1) , 14
- https://doi.org/10.1186/1475-2859-7-14
Abstract
Introducing point mutations into bacterial chromosomes is important for further progress in studies relying on functional genomics, systems- and synthetic biology, and for metabolic engineering. For many investigations, chromosomal systems are required rather than artificial plasmid based systems.Keywords
This publication has 27 references indexed in Scilit:
- Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineeringNucleic Acids Research, 2007
- An Improved Recombineering Approach by Adding RecA to λ Red RecombinationMolecular Biotechnology, 2006
- The Contribution of a Novel Ribosomal S12 Mutation to Aminoglycoside Resistance ofEscherichia coliMutantsJournal of Chemotherapy, 2004
- The Roles and Regulation of Potassium in BacteriaPublished by Elsevier ,2004
- Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cellsBMC Molecular Biology, 2003
- One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR productsProceedings of the National Academy of Sciences, 2000
- Does the KdpA Subunit from the High Affinity K+-Translocating P-Type KDP-ATPase have a Structure Similar to That of K+ Channels?Biophysical Journal, 2000
- A new logic for DNA engineering using recombination in Escherichia coliNature Genetics, 1998
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976