Selective Inhibition of IgE versus beta2-Microglobulin in Human U-266 Myeloma Cell Line Treated with T-Cell-Derived Factors

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Abstract
Concanavalin-A-activated T cells and their crude supernatants were assayed for suppressive activity on an IgE-producing U-266 cell line. Detectable and comparable degrees of suppression were obtained with the co-culture and the supernatant protocols. Separation of the effector population into T4+ and T8+ subsets showed the most effective cells in the T8+ fraction. Control experiments demonstrated that the IgE down-regulation was selective, since parallel measurement of .beta.2-microglobulin synthesis showed no effect of T cells or T cell-derived supernatants. In addition, several human T cell lymphoma-leukemia virus I-transformed T cell lines were explored for their capacity to produce factor(s) able to suppress IgE synthesis in the U-266 cell line, and 4 out of 25 cell lines could be shown to do this in a constitutive manner. Kinetic studies suggested that the inhibition occurred at a transcription level. Apparently, the T cell-myeloma system is an interesting model to define better the regulation of IgE in the human.
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