The Pto Bacterial Resistance Gene and the Fen Insecticide Sensitivity Gene Encode Functional Protein Kinases with Serine/Threonine Specificity
- 1 August 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 108 (4) , 1735-1739
- https://doi.org/10.1104/pp.108.4.1735
Abstract
The catalytic activity and amino acid specificity of the tomato Pto and Fen kinases were investigated. The Pto and Fen genes were fused to the carboxyl terminus of the maltose-binding protein and expressed in Escherichia coli. Incubation of the purified fusion proteins with [gamma-32P]ATP in an in vitro assay showed that both proteins were capable of autophosphorylation. Mutant fusion proteins in which the conserved lysine residue of subdomain II was changed to a glutamine were unable to autophosphorylate. Phosphoamino analysis of the active fusion proteins indicated that both kinases phosphorylate serine and threonine residues but not tyrosine.Keywords
This publication has 26 references indexed in Scilit:
- A member of the tomato Pto gene family confers sensitivity to fenthion resulting in rapid cell death.Plant Cell, 1994
- Biochemical properties of the autophosphorylation of RLK5, a receptor-like protein kinase from Arabidopsis thalianaBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1994
- Receptor‐like protein kinase genes of Arabidopsis thalianaThe Plant Journal, 1993
- A Plant Receptor-Like Gene, the S-Locus Receptor Kinase of Brassica oleracea L., Encodes a Functional Serine/Threonine KinasePlant Physiology, 1993
- CTR1, a negative regulator of the ethylene response pathway in arabidopsis, encodes a member of the Raf family of protein kinasesCell, 1993
- The TMK1 gene from Arabidopsis codes for a protein with structural and biochemical characteristics of a receptor protein kinase.Plant Cell, 1992
- The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase.Plant Cell, 1992
- cDNA Sequence of PsPK5, a Protein Kinase Homolog from Pisum sativum L.Plant Physiology, 1992
- Relationship of a putative receptor protein kinase from maize to the S-locus glycoproteins of BrassicaNature, 1990
- An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding proteinGene, 1988