Na+-driven ATP synthesis inMethanobacterium thermoautotrophicumand its differentiation from H+-driven ATP synthesis by rhodamine 6G
- 27 June 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 347 (2-3) , 190-194
- https://doi.org/10.1016/0014-5793(94)00535-4
Abstract
Rhodamine 6G (3 μM) effectively inhibited ΔpH-driven ATP synthesis in Methanobacterium thermoautotrophicum while ΔpNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg2+-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detached from the membrane was insensitive to this inhibitor. Methanogenesis-driven ATP synthesis at pH 6.8 of cells grown in the presence of 50 mM NaCl was inhibited by rhodamine 6G both in the presence of 5 mM and 50 mM NaCl. On the other hand, the methanogenesis-driven ATP synthesis at pH 8.0 of cells grown in the presence of 50 mM NaCl was slightly inhibited by rhodamine 6G in the presence of 5 mM NaCl and was not inhibited at all in the presence of 50 mM NaCl. The growth experiments have shown that cells of Methanobacterium thermoautotrophicum can grow under alkaline conditions even in the presence of rhodamine 6G and of high NaCl concentration when the growth media were inoculated with the cells which had been grown in the presence of 50 mM NaCl. These results indicate that sodium-motive force-driven ATP synthase in Methanobacterium thermoautotrophicum operates effectively in alkaline conditions and it might be the sole ATP synthesizing system when the proton-motive force-supported ATP synthesis is inhibited by rhodamine 6G.Keywords
This publication has 26 references indexed in Scilit:
- Cytochrome d induction in Escherichia coli growing under unfavorable conditionsFEBS Letters, 1993
- A DNA fragment homologous to F1‐ATPase β subunit was amplified from genomic DNA of Methanosarcina barkeri Indication of an archaebacterial F‐type ATPaseFEBS Letters, 1992
- Chemiosmotic systems in bioenergetics: H+-cycles and Na+-cyclesBioscience Reports, 1991
- Primary structure of the α‐subunit of vacuolar‐type Na+‐ATPase inEnterococcus hirae Amplification of a 1000‐bp fragment by polymerase chain reactionFEBS Letters, 1991
- Amplification of the Na+-ATPase ofStreptococcus faecalisat alkaline pHFEBS Letters, 1990
- Na+-driven ATP synthesis inMethanobacterium thermoautotrophicumcan be modulated with sodium ion concentrations in the growth mediumFEBS Letters, 1988
- Discrimination between transmembrane ion gradient‐driven and electron transfer‐driven ATP synthesis in the methanogenic bacteriaFEBS Letters, 1986
- An electrogenic sodium‐translocating ATPase in Methanococcus voltaeFEBS Letters, 1986
- Sodium bioenergeticsTrends in Biochemical Sciences, 1984
- Sodium‐dependent isoleucine transport in the methanogenic archaebacterium Methanococcus voltaeFEBS Letters, 1984