Evidence that rnmB is the operator of the Escherichia coli recA gene.
- 1 March 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (3) , 1786-1790
- https://doi.org/10.1073/pnas.78.3.1786
Abstract
The rnmB281 mutation leads to high constitutive levels of recA protein such that no increase after uninducing treatment occurs. The mutation maps in or near the portion of recA corresponding to the NH2-terminal end of the protein. Examination of the recA proteins from rnmB+ recA-/rnmB281 recA+ heterozygotes suggests that both rnmB alleles are cis-acting and codominant. This is the behavior expected from alleles of a regulatory gene such as an operator or promoter of recA. The possibility that rnmB mutations occur in the promoter of recA, though not ruled out, seems unlikely based on the structure of the regulatory region of recA. rnmB mutations may be operator constitutive mutations of the recA gene and should be called recAo mutations. The UV-irradiation responses of recAo+ and recAo281 strains, both recA+, are compared and inferences are drawn about the roles of large amounts of recA protein in producing the responses.This publication has 26 references indexed in Scilit:
- DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.Proceedings of the National Academy of Sciences, 1980
- Sequences of the recA gene and protein.Proceedings of the National Academy of Sciences, 1980
- Linkage map of Escherichia coli K-12, edition 6.1980
- E. coli recA protein-directed cleavage of phage λ repressor requires polynucleotideNature, 1980
- Organization of the recA gene of Escherichia coli.Proceedings of the National Academy of Sciences, 1980
- Indirect and intragenic suppression of the lexA102 mutation in E. coli B/rMolecular Genetics and Genomics, 1979
- W-reactivation of phage lambda in recF, recL, uvrA, and uvrB mutants of E. coli K-12Molecular Genetics and Genomics, 1979
- A Role for recF in Repair of UV Damage to DNAPublished by Cold Spring Harbor Laboratory ,1979
- Identification of the recA ( tif ) gene product of Escherichia coliProceedings of the National Academy of Sciences, 1977
- Protein X is the product of the recA gene of Escherichia coli.Proceedings of the National Academy of Sciences, 1977