Hydroxylation of aromatic compounds by reduced nicotinamide-adenine dinucleotide and phenazine methosulphate requires hydrogen peroxide and hydroxyl radicals, but not superoxide
- 1 October 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 167 (1) , 317-320
- https://doi.org/10.1042/bj1670317
Abstract
A mixture of NADH and phenazine methosulfate [which may represent a model system for oxygenase enzymes] hydroxylates aromatic compounds at acidic pH values. Hydroxylation is inhibited by catalase and by scavengers of the hydroxyl radical .**GRAPHIC**. but not by superoxide dismutase [EC 1.15.1.1]. Apparently, neither .**GRAPHIC**. is sufficiently reactive to hydroxylate aromatic rings.This publication has 13 references indexed in Scilit:
- An attempt to demonstrate a reaction between superoxide and hydrogen peroxideFEBS Letters, 1976
- Oxidation of manganous pyrophosphate by superoxide radicals and illuminated spinach chloroplastsArchives of Biochemistry and Biophysics, 1976
- Hydroxylation of p‐Coumaric Acid by Illuminated ChloroplastsEuropean Journal of Biochemistry, 1975
- Mechanism of aromatic hydroxylation : involvement of perhydroxyl radicals and semiquinones in hydroxylation of aromatic amino acids by a model system.1975
- Studies on nucleotidases in plantsArchives of Biochemistry and Biophysics, 1975
- Mechanism of aromatic hydroxylationArchives of Biochemistry and Biophysics, 1974
- On the development of a well‐defined source of superoxide ion for studies with biological systemsFEBS Letters, 1974
- Superoxide dismutase: a contaminant of bovine catalase (Short Communication)Biochemical Journal, 1973
- Mechanism of hydroxylation of aromatic compounds: Evidence for the involvement of superoxide anion in a model systemBiochemical and Biophysical Research Communications, 1972
- The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygenBiochemical and Biophysical Research Communications, 1972