Control of the ppp(A2′p)nA System in HeLa Cells
- 1 May 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 124 (1) , 131-138
- https://doi.org/10.1111/j.1432-1033.1982.tb05915.x
Abstract
HeLa cells have an unusually high level of ppp(A2′p)nA synthetase(n = 2 to ≥4) even in the absence of interferon treatment. In accord with this ppp(A2′p)nA and ppp(A2′p)nA‐mediated ribosomal RNA cleavage occur naturally in response to encephalomyocarditis virus infection in control as well as in intcrleron‐treated cells. Despite this, in the absence of interferon treatment, encephalomyocarditis virus grows well In these cells. A possible explanation for this paradox is that the ppp(A2′p)nA dependent RNase is lost or inactivated at later times post‐infection in control but not in interferon‐treated cells. It appears, therefore, to bc the prevention by interferon of the virus‐mediated inhibition of the ppp(A2′p)n‐dependent nuclease rather than the absolute level or induction of the ppp(A2′p)nA synthetase which is crucial for the activity of the ppp(A2′p)nA system in HeLa cells. These results provide evidence for a further level of control in the ppp(A2′p)nA system and show that limited ppp(A2′p)nA‐mediated ribosomal RNA cleavage alone is not sufficient to cause;in inhibition of virus growth.This publication has 33 references indexed in Scilit:
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