THE PHYSIOLOGICAL RESPONSE TO ENTEROCRININ CONSIDERED QUANTITATIVELY

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Abstract
A detailed procedure is given for the bioassay of enterocrinin, the hormone which excites the secretory glands of the intestine. The principal feature of the method, which permits accurate and reproducible results to be obtained quickly from the highly variable intestinal secretory response, is its precise treatment of the time factor in the normal variations in responsiveness. The general procedure outlined may well be of value in increasing the accuracy and practicability of other types of bioassays. An avg. curve of response to enterocrinin was used to assist in equilibrating the responses to the standard and the "unknown" preps. A statistical analysis of data on 9 dogs showed a closer dose: response correlation when the dose of enterocrinin was given without reference to the wt. of the dog than when it was given on a per kg. wt. basis. Secretin, likewise, gave more uniform responses in different dogs if the same dose were given, regardless of body wt., while the vasodilatins present in intestinal extracts were best expressed on a per kg. basis.