The use of Ultroser G as a serum substitute in the culture of human skin fibroblasts
- 1 January 1986
- journal article
- research article
- Published by Wiley in Biology of the Cell
- Vol. 57 (3) , 243-247
- https://doi.org/10.1111/j.1768-322x.1986.tb00480.x
Abstract
Growth of human skin fibroblasts was dramatically enhanced when serum in the culture medium was replaced by Ultroser G. Compared to the same cells cultured in the presence of serum, alterations in glucose and lipid metabolism and an increase in the activity of prolidase (EC 3.4.13.9) and prolinase (EC 3.4.13.8) were also observed. Consequently, we advise extreme caution in the use of Ultroser G in metabolic studies, especially for periods of culture exceeding 10 days. However, Ultroser G can help to produce a large number of cells and so facilitate purification of the proteins produced during the stationary growth phase.This publication has 4 references indexed in Scilit:
- Relationship between cell density and prolidase activity in human skin fibroblasts: effects of ascorbate and fructoseBiochimie, 1984
- Culture of chondrocytes in medium supplemented with fetal calf serum or a serum substitute: Ultroser GBiology of the Cell, 1984
- Effects of pexid on liver cell cultures ultrastructural and histoenzymological studiesEuropean Journal of Clinical Pharmacology, 1981
- Some metabolic differences between human skin and aponeurosis fibroblasts in culutreJournal of Cellular Physiology, 1980