Culture of chondrocytes in medium supplemented with fetal calf serum or a serum substitute: Ultroser G
- 1 January 1984
- journal article
- research article
- Published by Wiley in Biology of the Cell
- Vol. 51 (3) , 307-313
- https://doi.org/10.1111/j.1768-322x.1984.tb00309.x
Abstract
Fetal calf serum and a serum substitute, Ultroser G, were compared for their effects on the growth curves, clonal growth and cell cycle progression of rabbit chondrocytes in primary culture and during at least 3 cell passages and included a screen for the maintenance of cartilage-like differentiation, i.e., the presence of type II collagen. Proliferation was also compared with another serum substitute, Nu-Serum. Ultroser G is shown to be equivalent to fetal calf serum as far as chondrocyte proliferation is concerned, clonal growth is improved and biosynthesis of type II collagen is maintained in primary culture.This publication has 16 references indexed in Scilit:
- Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid-enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone.Proceedings of the National Academy of Sciences, 1981
- Multiplication of human diploid fibroblasts in a synthetic medium supplemented with EGF, insulin, and dexamethasoneExperimental Cell Research, 1981
- Growth regulation of WI38 cells in a serum-free mediumExperimental Cell Research, 1981
- Complete replacement of serum in primary cultures of erythropoietin-dependent red cell precursors (CFU-E) by albumin, transferrin, iron, unsaturated fatty acid, lecithin and cholesterolExperimental Cell Research, 1980
- A serum-free medium supplemented with multiplication-stimulating activity (MSA) supports both proliferation and differentiation of chondrocytes in primary cultureExperimental Cell Research, 1980
- The use of hormone-supplemented serum-free media in primary culturesExperimental Cell Research, 1979
- Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.Proceedings of the National Academy of Sciences, 1979
- Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.Proceedings of the National Academy of Sciences, 1978
- The progeny of rabbit articular chondrocytes synthesize collagen types I and III and type I trimer, but not type II. Verifications by cyanogen bromide peptide analysisBiochemistry, 1977
- Metabolism of cells in tissue culture in vitroExperimental Cell Research, 1961