Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2) , 901-904
- https://doi.org/10.1073/pnas.75.2.901
Abstract
HeLa [human cervical cancer] cells grow in a nutritionally complete synthetic medium (Ham''s F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation and a growth rate equal to that of serum-supplemented medium. The omission of any 1 of the 5 components results in less than maximal cell growth.This publication has 18 references indexed in Scilit:
- Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.Proceedings of the National Academy of Sciences, 1976
- Epidermal Growth Factor: Chemical and Biological Characterization,Published by Elsevier ,1974
- Pituitary Extracts and Steroid Hormones in the Control of 3T3 Cell GrowthProceedings of the National Academy of Sciences, 1973
- Growth Response to Hormones by a New Rat Ovary Cell LineNature New Biology, 1972
- Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic mediaExperimental Cell Research, 1971
- CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUMProceedings of the National Academy of Sciences, 1965
- Chemically defined media for cultivation of long-term cell strains from four mammalian speciesExperimental Cell Research, 1964
- Nutrition Needs of Mammalian Cells in Tissue CultureScience, 1955
- A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORSProceedings of the National Academy of Sciences, 1955
- THE SPECIFIC AMINO ACID REQUIREMENTS OF A HUMAN CARCINOMA CELL (STRAIN HELA) IN TISSUE CULTUREThe Journal of Experimental Medicine, 1955