CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM
- 1 February 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 53 (2) , 288-293
- https://doi.org/10.1073/pnas.53.2.288
Abstract
A chemically defined, synthetic medium was developed which will support both single cell plating and serial propagation of 2 Chinese hamster cell lines. With slight modification, the medium will also support both types of growth for a strain of mouse L cells.Keywords
This publication has 17 references indexed in Scilit:
- Preparation of Mammalian Cell Cultures with Enzyme from Aspergillus oryzae.Experimental Biology and Medicine, 1963
- Biotin, B12, and other vitamin requirements of a strain of mammalian cells grown in chemically defined mediumExperimental Cell Research, 1963
- Albumin Replacement by Fatty Acids in Clonal Growth of Mammalian CellsScience, 1963
- Clonal growth of diploid Chinese hamster cells in a synthetic medium supplemented with purified protein fractionsExperimental Cell Research, 1962
- FURTHER STUDIES ON AMINO ACID REQUIREMENTS OF A SUBLINE OF STRAIN L CELLS (MOUSE FIBROBLASTS) IN SYNTHETIC MEDIA1961
- MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLSThe Journal of Experimental Medicine, 1959
- Rapid Proliferation of Sublines of NCTC Clone 929 (Strain L) Mouse Cells in a Simple Chemically Defined Medium (MB 752/1)2JNCI Journal of the National Cancer Institute, 1959
- GENETICS OF SOMATIC MAMMALIAN CELLSThe Journal of Experimental Medicine, 1958
- The Effect of Inoculum Size on Proliferation of NCTC Strain 2071, the Chemically Defined Medium Strain of NCTC Clone 929 (Strain L)JNCI Journal of the National Cancer Institute, 1958
- Nutrition of Animal Cells in Tissue Culture. X. Synthetic Medium No. 858,Experimental Biology and Medicine, 1955