Rapid Proliferation of Sublines of NCTC Clone 929 (Strain L) Mouse Cells in a Simple Chemically Defined Medium (MB 752/1)2

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Abstract
A chemically defined nutrient solution containing 40 components is described. In this solution, cells of sublines of NCTC clone 929 (strain L) mouse cells have been maintained for more than 30 passages in rapid proliferative growth, with the cell population increasing 7.5-fold to 8.5-fold (from an inoculum of 2 to 3 × 105 cells per ml.) in an 8-day period.