A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS
- 15 July 1955
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 41 (7) , 432-437
- https://doi.org/10.1073/pnas.41.7.432
Abstract
A simple method for production of clones from almost all the cells of HeLa population is described. The technique can be used to titrate the number of cells capable of reproduction in a suspension and should find application in genetic and metabolic studies involving the action on animal cells of viruses, drugs, nutritional stresses, and mutagenic agents.Keywords
This publication has 4 references indexed in Scilit:
- CERTAIN FACTORS LIMITING THE SIZE OF THE TISSUE CULTURE AND THE DEVELOPMENT OF MASSIVE CULTURESAnnals of the New York Academy of Sciences, 1954
- Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.Experimental Biology and Medicine, 1954
- PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSESThe Journal of Experimental Medicine, 1954
- Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium.,Experimental Biology and Medicine, 1950