PLATELET PRESERVATION. I. PRESERVATION OF CANINE PLATELETS AT 4° C*

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Abstract
Canine platelets have a 7 to 8 day lifespan by the Cr51 labeling method using either ACD or EDTA anticoagulants. Platelet sequestration was observed in that the platelet bound radioactivity was lower on the 1/2 hour than on the 2 hour sample. The downslope of platelet bound radio-activity was curvilinear. The Cr51 platelet lifespan was used as the major criterion of platelet viability in storage experiments. Platelet concentrates derived from platelet rich plasma stored at 4[degree]C show a progressive diminution in viability with increasing periods of storage beyond 2 hours with virtually complete loss of viability after 12 hours storage. Platelet concentrates stored at 4[degree]C as such showed a progressive diminution in viability with increasing periods of storage beyond 2 hours with virtually complete loss of viability after 7 hours storage. Clot retraction was maintained in platelets stored as platelet rich plasma for periods up to 54 hours indicating a discrepancy between the parametersof in vivo viability by Cr51 measurement and clot retraction.