Comparison of the Anthrone Reagent and a Copper-Reduction Method for Determining Blood Sugar in Calves
Open Access
- 1 November 1961
- journal article
- research article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 44 (11) , 2081-2088
- https://doi.org/10.3168/jds.s0022-0302(61)90022-4
Abstract
Protein-free filtrates from 1500 samples of blood from 12 calves were prepared according to Folin-Wu. An aliquot of each filtrate was analyzed for blood sugar by both the anthrone reagent and the Somogyi-Nelson technique. The anthrone procedure is as follows: pipet one ml of sample filtrate, blank, or standard into a test tube and chill; add 10 ml of ice-cold 0.05% anthrone dissolved in 72% sulfuric acid to each test tube while immersed in ice water, and mix. After equalizing the temperature of the tubes in a tap-water bath, place them in a boiling water bath for 10 minutes; cool in a running tap-water bath. Pour the reactants into matched spectrophotometer cuvettes and read at a wavelength of 620 mu. after aging for 1/2 hour in the dark. Statistical analysis indicated that the difference between the two techniques was highly significant. The anthrone values were less than 5% greater. The correlation coefficient between the results of the two methods was 0.94. The inherent error in both methods was the same, as indicated by almost identical standard deviations.This publication has 20 references indexed in Scilit:
- A Micromethod for Simultaneous Determination of Glucose and Ketone Bodies in Blood and Glycogen and Ketone Bodies in LiverScandinavian Journal of Clinical and Laboratory Investigation, 1960
- Dependence of Aqueous-Blood Sugar Ratios on Method of DeterminationArchives of Ophthalmology (1950), 1959
- Anthrone ReagentAnalytical Chemistry, 1956
- Factors Influencing Quantitative Determination of Methylpentoses and Ketohexoses with AnthroneAnalytical Chemistry, 1955
- STOMATAL MOVEMENTS AND CHANGES OF CARBOHYDRATE IN LEAVES OF CHRYSANTHEMUM MAXIMUMNew Phytologist, 1954
- Anthrone in Estimation of Hexose Sugars with Special Reference to Pentose InterferenceAnalytical Chemistry, 1954
- Determination of Dextran with AnthroneAnalytical Chemistry, 1953
- Determination of Starch and Amylose in VegetablesAnalytical Chemistry, 1950
- Rapid Measurement of Carbohydrate in Blood: Preliminary ReportPublic Health Reports (1896-1970), 1950
- Qualitative Test for Carbohydrate MaterialIndustrial & Engineering Chemistry Analytical Edition, 1946