A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
Open Access
- 1 April 1982
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 1 (4) , 415-420
- https://doi.org/10.1002/j.1460-2075.1982.tb01184.x
Abstract
We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta‐galactosidase gene of a M13‐lac vector, generally causing loss of beta‐galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta‐galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.This publication has 36 references indexed in Scilit:
- DIRECTED MUTAGENESISAnnual Review of Genetics, 1981
- A split promoter for a eucaryotic tRNA geneCell, 1981
- Transcriptional control regions of the adenovirus VAI RNA geneCell, 1980
- The influence of codon context on genetic code translationNature, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Expression of sea urchin histone genes in the oocyte of Xenopus laevisJournal of Molecular Biology, 1979
- A fast and simple method for sequencing DNA cloned in the single-stranded bacteriophage M13Journal of Molecular Biology, 1979
- Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sitesNature, 1978
- Deoxyribonuclease I produces staggered cuts in the DNA of chromatinJournal of Molecular Biology, 1977