Growth stimulative effect of parathyroid hormone, calcitonin and N6,O2'-dibutyryl adenosine 3';5'-cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium.

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Abstract
PTH [parathyroid hormone] (0.5 U/ml) and CT [calcitonin] (0.5 U/ml) strongly stimulated the in vitro growth of the chick embryo femur in terms of elongation, dry weight increase and an increase in the protein, hexosamine, hydroxyproline, DNA and RNA content of the femur. A synergistic effect was observed when PTH and CT were simultaneously added to the medium; the mechanism of the growth stimulating effect of one of these hormones may be different from that of the other. The exposure of PTH to the femur caused an increase in cAMP content. The direct addition of dibutyryl cAMP (1 mM) to the medium mimicked the effect of PTH on the cartilage. PTH seems to stimulate the growth of cartilage via the increase in cAMP content of the femur. CT had no effect on the cAMP content of the femur.