Uptake of auxotrophic cells of a heterocyst-forming cyanobacterium by tobacco protoplasts, and the fate of their associations
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 139 (1) , 55-60
- https://doi.org/10.1007/bf00390810
Abstract
Auxotrophic cells of the filamentous cyanobacterium Anabaena variabilis Kütz. were introduced into protoplasts of Nicotiana tabacum L. in an attempt to engineer a nitrogen-fixing endosymbiosis. Conditions were established to maximize uptake of the cyanobacteria by use of polyethylene glycol. Culture of the novel association was not successful: tobacco protoplasts with Anabaena inside did not appear to divide. Most of the protoplasts expelled the cyanobacteria and simultaneously disintegrated. The reasons for the incompatibility are not known.This publication has 18 references indexed in Scilit:
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