Heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium
- 1 November 1976
- journal article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 110 (2) , 145-147
- https://doi.org/10.1007/bf00690221
Abstract
Anabaena variabilis can be cloned in the dark from fragments with one and few cells, with an efficiency of about 40%, on the nitrogen-free medium of Allen and Arnon solidified with 0.5% agarose and supplemented with 5 mM fructose. The organism can be grown exponentially (τ2≅36 h) in fermentor cultures in the dark, fixing N2, to a density of greater than 10 g dry weight/l.Keywords
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