Spodoptera frugiperda Nuclear Polyhedrosis Virus Genome: Physical Maps for Restriction Endonucleases Bam HI and Hin dIII
- 1 June 1981
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 38 (3) , 922-931
- https://doi.org/10.1128/jvi.38.3.922-931.1981
Abstract
The physical map for the genome of Spodoptera frugiperda nuclear polyhedrosis virus was constructed for restriction endonucleases Bam HI and Hin dIII. The ordering of the restriction fragments was accomplished by cross-blot hybridization of Bam HI, Hin dIII, and Eco RI fragments. The alignment of the Hin dIII fragments within the Bam HI map was achieved by double digestion with the two restriction endonucleases followed by cross-blot hybridization. The results showed that the viral genome consisted of mainly unique sequences. In addition, the circular nature of the viral genome was reaffirmed.This publication has 18 references indexed in Scilit:
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