The Bioassay of Insect Feeding Tokens

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Abstract
The acceptability of host plant extracts on pith, charred filter paper, and an agar diet, and vacuum infiltrated into lettuce leaves was compared on three species of Lepidoptera and two of Coleoptera. The vacuum infiltrated lettuce was eaten by two of the Lepidoptera and it was the only method acceptable to the Coleoptera. The agar diet was eaten by the three Lepidoptera while pith and charred filter paper were acceptable to only two of them. It is concluded that vacuum infiltration of lettuce leaves is a useful method for the bioassay of host plant extracts to determine if they contain insect feeding token substances.