Fraktionierung der nicht-kollagenen Komponenten des Gelenkkapselbindegewebes

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Abstract
The joint-capsule of calf contained 74% collagen, 15.5% of non-collagenic proteins and peptides respectively, 2-2.5% carbohydrates and lipids. Two percent of the collagen was soluble. Elastin could not be detected. About 1/2 of the proteins from the ground substances were soluble in 0.15 and 0.45M NaCl (soluble proteins of the ground substance). From the tighter bound portion (non-collagenic structural proteins) 2 fractions could be isolated at pH 3 and pH 9 respectively, which contained 28-31% carbohydrates. After removing the collagenic matrix with hot trichloro-acetic acid a large amount of soluble proteins containing only a little carbohydrate could be obtained. Altogether 82% of the non-collagenic N could be solubilized, of which about 1B was dialysable. Only 0.15% of the tissue could not be solubilized.[long dash]Author.