A panel of repeat markers for detection of microsatellite instability in murine tumors
- 19 November 2003
- journal article
- research article
- Published by Wiley in Molecular Carcinogenesis
- Vol. 38 (4) , 155-159
- https://doi.org/10.1002/mc.10157
Abstract
A substantial fraction of human malignancies lack functional DNA mismatch repair (MMR), accumulate mutations at high frequency, and exhibit microsatellite instability (MSI). In order to distinguish between MMR‐competent and MMR‐deficient malignancies, a consensus panel of microsatellite repeats has been adopted for MSI analysis of human tumors. There is no reference panel of repeats for MSI typing of murine malignancies. In this study, we present six new microsatellite repeat markers for MSI typing of mouse tumors. Analysis of polymerase chain reaction (PCR)‐amplified tumor DNA from MMR‐deficient and ‐proficient mice on denaturing polyacrylamide gels revealed that the new panel of microsatellites was more sensitive in detecting MSI than six commonly used CAn repeats. Using the new set of microsatellite markers, we demonstrated the presence of MSI in endometrial carcinoma and cancer precursors from diethylstilbestrol (DES)‐treated mice, pointing to a possible role for loss of MMR in hormonally promoted endometrial tumorigenesis.Keywords
This publication has 13 references indexed in Scilit:
- Expression Profiling of Mouse Endometrial Cancers Microdissected from Ethanol-Fixed, Paraffin-Embedded TissuesThe American Journal of Pathology, 2003
- Multiple mutations and cancerProceedings of the National Academy of Sciences, 2003
- Evidence for sequential action of two ATPase active sites in yeast Msh2–Msh6DNA Repair, 2002
- Stepwise Deletions of PolyA Sequences in Mismatch Repair-Deficient Colorectal CancersThe American Journal of Pathology, 2001
- Efficient detection of hereditary nonpolyposis colorectal cancer gene carriers by screening for tumor microsatellite instability before germline genetic testingGastroenterology, 2001
- Tumour susceptibility and spontaneous mutation in mice deficient in Mlh1, Pms1 and Pms2 DMA mismatch repairNature Genetics, 1998
- Female embryonic lethality in mice nullizygous for both Msh2 and p53Nature Genetics, 1997
- Meiotic Pachytene Arrest in MLH1-Deficient MiceCell, 1996
- Microsatellite Instability in Cancer of the Proximal ColonScience, 1993
- Clues to the Pathogenesis of Familial Colorectal CancerScience, 1993